Difference between revisions of "Conformational Switching"
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== Experiments == |
== Experiments == |
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| + | * Self-assembly experiment at different speeds: |
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| − | * Setting of the speed for experiments |
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** Result: self-assembly time distribution for each speed; use in experiments speed with lowest average time to completion + 2 std. dev. |
** Result: self-assembly time distribution for each speed; use in experiments speed with lowest average time to completion + 2 std. dev. |
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| − | * Test to verify role of seed |
+ | * Test to verify role of seed: |
** Passive components, no seed |
** Passive components, no seed |
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** Passive components, AND seed |
** Passive components, AND seed |
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=== Particulars of experiments (10 trials each) === |
=== Particulars of experiments (10 trials each) === |
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| + | * Self-assembly experiment at different speeds: |
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| − | * Run each experiment till self-assembly of one target structure |
+ | ** Run each experiment till self-assembly of one target structure |
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| + | *** All components are seeds |
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| + | *** Passive components, no seed |
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| + | *** Passive components, AND seed |
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| ⚫ | |||
* Assembly of one target structure (Data to be collected: number of individual components, number of compatible substructures, number of incompatible substructures, number of targets) |
* Assembly of one target structure (Data to be collected: number of individual components, number of compatible substructures, number of incompatible substructures, number of targets) |
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| Line 38: | Line 42: | ||
** Scaling |
** Scaling |
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** Removing the acceleration rump-up phase |
** Removing the acceleration rump-up phase |
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| + | ** Plot of Gibbs free energy |
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== Meeting minutes == |
== Meeting minutes == |
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Revision as of 18:29, 24 October 2014
Schedule
- 1st December, 2014 : Submit final draft of Conformational Switching paper to Marco
- 22nd November, 2014 : Finish first draft of Conformational Switching paper
- 8th November, 2014 : Finalise results, create plots
- 1st November, 2014 : Finalise plan for experiments and collect preliminary data
Todo
- Outline by 8th November
- Mount existing container on shaker
Experiments
- Self-assembly experiment at different speeds:
- Result: self-assembly time distribution for each speed; use in experiments speed with lowest average time to completion + 2 std. dev.
- Test to verify role of seed:
- Passive components, no seed
- Passive components, AND seed
Particulars of experiments (10 trials each)
- Self-assembly experiment at different speeds:
- Run each experiment till self-assembly of one target structure
- All components are seeds
- Passive components, no seed
- Passive components, AND seed
- Use different speeds starting from lowest speed (= when components start to move) and increment speed by 25 rpm till target structure breaks.
- Run each experiment till self-assembly of one target structure
- Assembly of one target structure (Data to be collected: number of individual components, number of compatible substructures, number of incompatible substructures, number of targets)
- All components are seeds
- Passive components, no seed
- Passive components, AND seed
- Assembly of two target structures (Data to be collected: number of individual components, number of compatible substructures, number of incompatible substructures, number of targets)
- All components are seeds
- Passive components, no seed
- Passive components, AND seed
- Video recording of experiments, probably one frame every seconds.
- Results to show
- Experiments for setting parameter (shaking speed)
- Time evolution of self-assembly
- Conf switch eliminates incompatible structures
- Scaling
- Removing the acceleration rump-up phase
- Plot of Gibbs free energy
