Difference between revisions of "Conformational Switching"

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* Assembly of one target structure (Data to be collected: number of individual components, number of compatible substructures, number of incompatible substructures, number of targets)
 
* Assembly of one target structure (Data to be collected: number of individual components, number of compatible substructures, number of incompatible substructures, number of targets)
  +
** The results of the experiment where all the components are seeds and the experiment where there are passive components and a seed ''should have the same yield''
 
** All components are seeds
 
** All components are seeds
 
** Passive components, no seed
 
** Passive components, no seed
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* Assembly of two target structures (Data to be collected: number of individual components, number of compatible substructures, number of incompatible substructures, number of targets)
 
* Assembly of two target structures (Data to be collected: number of individual components, number of compatible substructures, number of incompatible substructures, number of targets)
  +
** When there are components (only seeds) enough to make more than one target structure, the incompatible substructure problem is observed
 
** All components are seeds
 
** All components are seeds
 
** Passive components, no seed
 
** Passive components, no seed
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** Removing the acceleration rump-up phase
 
** Removing the acceleration rump-up phase
 
** Plot of Gibbs free energy
 
** Plot of Gibbs free energy
  +
*** Gibbs free energy related to the number of available bonding sites.
   
 
== Meeting minutes ==
 
== Meeting minutes ==

Revision as of 18:29, 24 October 2014

Schedule

  • 1st December, 2014 : Submit final draft of Conformational Switching paper to Marco
  • 22nd November, 2014 : Finish first draft of Conformational Switching paper
  • 8th November, 2014 : Finalise results, create plots
  • 1st November, 2014 : Finalise plan for experiments and collect preliminary data

Todo

  • Outline by 8th November
  • Mount existing container on shaker

Experiments

  • Self-assembly experiment at different speeds:
    • Result: self-assembly time distribution for each speed; use in experiments speed with lowest average time to completion + 2 std. dev.
  • Test to verify role of seed:
    • Passive components, no seed
    • Passive components, AND seed

Particulars of experiments (10 trials each)

  • Video recording of experiments, probably one frame every second (1 fps).
  • Self-assembly experiment at different speeds:
    • Run each experiment till self-assembly of one target structure
      • All components are seeds
      • Passive components, no seed
      • Passive components, AND seed
    • Use different speeds starting from lowest speed (= when components start to move) and increment speed by 25 rpm till target structure breaks.
  • Assembly of one target structure (Data to be collected: number of individual components, number of compatible substructures, number of incompatible substructures, number of targets)
    • The results of the experiment where all the components are seeds and the experiment where there are passive components and a seed should have the same yield
    • All components are seeds
    • Passive components, no seed
    • Passive components, AND seed
  • Assembly of two target structures (Data to be collected: number of individual components, number of compatible substructures, number of incompatible substructures, number of targets)
    • When there are components (only seeds) enough to make more than one target structure, the incompatible substructure problem is observed
    • All components are seeds
    • Passive components, no seed
    • Passive components, AND seed
  • Results to show
    • Experiments for setting parameter (shaking speed)
    • Time evolution of self-assembly
    • Conformational switching eliminates incompatible structures
    • Scaling
    • Removing the acceleration rump-up phase
    • Plot of Gibbs free energy
      • Gibbs free energy related to the number of available bonding sites.

Meeting minutes

Meeeting 1 whiteboard capture
Meeeting 2 whiteboard capture